Margic protein marker western blot ladder
![margic protein marker western blot ladder margic protein marker western blot ladder](https://www.lubio.ch/assets/_processed_/9/3/csm_LU5001_AcuteBand_Prestained_Protein_Ladder_e576f5e5c1.png)
Does this marker contain Formaldehyde or Formamide? We do not have information regarding the prestained dye interfering with Bio-rad gel systems or the fluorescent dye associated with it. Can the tryptophane been tagged with the trihalo compounds of the biorad gels or the fluorescent dye? – 10 μl per well for large gels, 6 μl per well for blotsįAQ Which amino acids are linked to the BlueStar Plus Prestained Marker MWP04?Īmino acids involved in the dye reaction are those with amine side chain, such as Arg and Lys. – 5 μl per well for mini-gels, 3 μl per well for blots Load the following volumes of the ladder on SDS-polyacrylamide gel:.Mix thoroughly, to ensure that the solution is homogeneous.Thaw the ladder either at room temperature or at 37-40☌ for a few minutes to dissolve precipitated solids.
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ContentsĪpproximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, 2 % SDS, 0,2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).ĥ00 µL Protein Marker sufficient for 100 mini gels or 50 large gels. migration rate, gel composition, pH-value (see FAQ for more information). This is due to factors of the gel/buffer system that affect the running behaviour of proteins, e.g. The molecular weight of the marker bands can vary depending on the gel/buffer system used. The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.įigure 1: Molecular weight of the marker bands when using different buffer systems. The BlueStar PLUS Prestained Protein Marker is a three color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 – 240 kDa. Details BlueStar PLUS Prestained Protein Marker